Nucleotide sequence of rice waxy gene
Author(s) -
Zongyang Wang,
Zhiliang Wu,
Yanyan Xing,
Fei-gin Zheng,
Xiaoli Guo,
Weiguo Zhang,
MengMin Hong
Publication year - 1990
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/18.19.5898
Subject(s) - biology , gene , genetics , nucleic acid sequence , sequence (biology) , base sequence
Rice waxy gene codes for the starch granule-bound starch synthase (EC 2.4.1.11) (1). To characterize its molecular structure, a rice {Oryza sativa subsp. japonica Hengfeng cultivar) genomic library was constructed in XEMBL-3 vector. Two overlapping genomic clones containing waxy gene sequence were screened and identified from this library by using maize waxy gene DNA as probe (kindly provided by Dr N.Fedoroff). The nucleotide sequences of rice waxy gene and its 5' and 3' flanking regions (5499 bp) are presented here. Alignment of nucleotide sequence of waxy gene from rice with that of maize (2) and barley (3) revealed the presence of 13 introns and 14 exons. The fulllength of waxy preprotein is 609 amino acid residues. ACKNOWLEDGEMENTS
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