Complete nucleotide sequence of a gene coding forAspergillus aculeatuscellulase (FI-CMCase)

FI-CMCase, endoglucanase (EC 3.2.1.4), is the one of the ACKNOWLEDGEMENTS cellulase complex produced in Aspergillus aculeatus No. F-50 _ . , , . , „ » j ^ n i £ m « m (1). We have cloned a 3.5 kbp Xbal genomic DNA fragment This work was supported in part by Grants-n>Aid (#01603539) in E. coti HB101 that hybridize to a random-primed cDNA probe for ScienUfic Research from the M.nistry of Education, Science of the FI-CMCase coding region (2). Nucleotide sequence of a a n d C u I t u r e > P1.6 kbp Smal-Xbal fragment of the insert was determined to REFERENCES dideoxy-sequencing method (3) from both strands as shown. The open reading frame encoding a deduced protein of 237 amino '• %TM£; ^ ^ ^ ^ •*» -<> M M ^ °acids containing signal sequence is interrupted by two introns. 2. Ooi.T., Shinmyo.A., Okada.H., Hara,s., lkenaka,T., Murao.S. and Arai.M. The 5'-flanking sequence contains a putative TATA box at 81 (1990) Curr. Genet, in press. base upstream of the initiation COdon. 3. Sanger.F., Nicklen.S. and Coulsen.A.R. (1977) Proc. Nail. Acad. Sci. USA 74, 5463-5467.