Nucleoticle sequence of a full-length cDNA (PST-1) for aryl sulfotransferase from rat liver

A full-length cDNA encoding an isozyme of aryl sulfotransferase (or phenol sulfotransferase, EC 2.8.2.1) was isolated from a Xgtll cDNA expression library constructed from poly(A) + RNA of a ten-week-old male Sprague-Dawley rat liver by screening with a rabbit antiserum raised against a purified pnitrophenol sulfotransferase. The nucleotide sequence of the cDNA (PST-1) consists of 1028 nucleotides and contains an open reading frame of 873 nucleotides encoding a 291 amino acid protein (Mr 33,909 Da). The PST-1 cDNA shows 59% and 61% sequence similarity at the nucleotide level to those of hydroxysteroid sulfotransferase (STa) (1) and bovine oestrogen sulfotransferase (OST) (2), respectively, and the deduced amino acid sequence of the cDNA shares similarity of 41% and 54% with those of STa and OST. In Northern blot, using the fulllength cDNA probes, a major band was detected in total RNA of both male and female rat livers.