Sequence of a tRNAGlyfromStreptomyces coelicolor | Zendy

J. Stefan Rokem | Zendy; Astrid Schön | Zendy; Dieter Söll | Zendy

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Sequence of a tRNA^GlyfromStreptomyces coelicolor

Author(s) -

J. Stefan Rokem,

Astrid Schön,

Dieter Söll

Publication year - 1990

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/18.13.3988

Subject(s) - biology , streptomyces coelicolor , transfer rna , streptomycetaceae , sequence (biology) , streptomyces , genetics , base sequence , actinomycetales , rna , dna , bacteria , gene

tRNA was isolated from S. coelicolor A3 (2) J650. The cells were grown for 48 h at 30°C and 150 rpm in AM medium (1), broken by N2 cavitation (2) and the lysate was passed directly into phenol, extracted and precipitated. tRNAs were deacylated, purified on DEAE cellulose and separated by RPC-5 chromatography (3). End labeled tRNAs were purified to homogeneity on a 20% polyacrylamide/8M urea gel, and sequenced by standard methods (4, 5).

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