Open AccessQuantitative evaluation ofEscherichia colihost strains for tolerance to cytosine methylation in plasmid and phage recombinants
Author(s) -
D.M. Woodcock,
P.J. Crowther,
J.P. Doherty,
Stacie Jefferson,
Exmond E. Decruz,
Mario Noyer-Weidner,
Steven S. Smith,
Michael Michael,
Michael W. Graham
Publication year - 1989
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/17.9.3469
Subject(s) - biology , plasmid , cloning (programming) , escherichia coli , dna methylation , methylation , recombinant dna , cytosine , genetics , dna , genome , molecular cloning , gene , gene expression , computer science , programming language
Many strains of E. coli K12 restrict DNA containing cytosine methylation such as that present in plant and animal genomes. Such restriction can severely inhibit the efficiency of cloning genomic DNAs. We have quantitatively evaluated a total of 39 E. coli strains for their tolerance to cytosine methylation in phage and plasmid cloning systems. Quantitative estimations of relative tolerance to methylation for these strains are presented, together with the evaluation of the most promising strains in practical recombinant cloning situations. Host strains are recommended for different recombinant cloning requirements. These data also provide a rational basis for future construction of 'ideal' hosts combining optimal methylation tolerance with additional advantageous mutations.
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