Sequence of cDNA for a novel light-induced glycin-rich protein | Zendy

Ralf Kaldenhoff | Zendy; Gerhard Richter | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Sequence of cDNA for a novel light-induced glycin-rich protein

Author(s) -

Ralf Kaldenhoff,

Gerhard Richter

Publication year - 1989

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/17.7.2853

Subject(s) - biology , complementary dna , sequence (biology) , peptide sequence , genetics , base sequence , sequence alignment , microbiology and biotechnology , computational biology , dna , gene

Irradiation with blue light induces chloroplast differentation in suspension cultured cells of Chenopodium rubrum (1). During this developmental process specific organelle proteins are synthesized de-novo. Concomitantly, a drastic rise in activity level and steady-state concentration of numerous mRNAs is observed (2). A cDNA library was prepared using poly(A)-rich RNA from cells exposed to blue light for 6-24h, and screened for clones which correspond to rapidly light-induced genes. Representative for these is clone HC1. Analysis of both strands of the 689 bp insert by dideoxy chain termination technique with T7 polymerase (3) revealed the complete coding sequence for a glycinrich protein of 144 amino acids which exhibits a domain structure: Residues 9 3 6 form a hydrophobic a-helix whereas the following sequence (37 113) consists of six repeats with two motifs each 6 amino acids long: -Gly-Tyr-Asn-Asn-Gly-Glyand -Gly-Tyr-His-Asn-Gly-Gly-.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research