A catalytic 13-mer ribozyme | Zendy

Alex C. Jeffries | Zendy; Robert H. Symons | Zendy

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A catalytic 13-mer ribozyme

Author(s) -

Alex C. Jeffries,

Robert H. Symons

Publication year - 1989

Publication title -

nucleic acids research

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/17.4.1371

Subject(s) - ribozyme , hairpin ribozyme , biology , hammerhead ribozyme , vs ribozyme , cleavage (geology) , mammalian cpeb3 ribozyme , rna , enzyme kinetics , dna , microbiology and biotechnology , stereochemistry , biochemistry , enzyme , gene , active site , chemistry , paleontology , fracture (geology)

A 13-mer oligoribonucleotide can act as a ribozyme for the specific self-cleavage of a 41-mer oligoribonucleotide substrate in the presence of Mg2+. The two sequences involved correspond to the self-cleavage hammerhead structure of the virusoid of lucerne transient streak virus. The Michaelis-menten kinetic parameters for the reaction were; Km 1.3 microM, Vmax 0.012 microM min-1, kcat 0.5 min-1. The 13-mer RNA is the smallest ribozyme so far reported. A DNA analogue of the 13-mer can not substitute for the RNA in the reaction.

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