Nucleotide sequence of the cDNA for murine intercellular adhesion molecul-1 (ICAM-1)

Department of Medicine, 'Howard Hughes Medical Institute and Institute for Molecular Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA Submitted June 6, 1989 EMBL accession no. X15372 We have isolated three unique cDNA clones from a mouse thymus cDNA library using a nucleotide probe derived from the human cDNA. These clones cover 2,522 bp with an open reading frame encoding for 532 amino acids beginning with ATG at bp 23 and ending with TGA at 1634. The identity between human and mouse amino acid sequence is 54% with a range from 48% in domains 1 and 5 ( by the immunoglobulin classification ) to 66% in domain 2. Two highly conserved regions are present in domain 2 flanked by RGD and RGE sequences which are potential tntegrin recognition sites. The identity between amino acid sequences of domains 1 and 2 of murine ICAM-1 and the extracellular domain of human ICAM-2 is 31% (human ICAM-1 versus human ICAM-2 is 34%).