Inhibition of DNA synthesis by cross-linking the template to platinum-thiol derivatives of complementary oligodeoxynucleotides | Zendy

Barbara C.F. Chu | Zendy; Leslie E. Orgel | Zendy

Open Access

Inhibition of DNA synthesis by cross-linking the template to platinum-thiol derivatives of complementary oligodeoxynucleotides

Author(s) -

Barbara C.F. Chu,

Leslie E. Orgel

Publication year - 1989

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/17.12.4783

Subject(s) - klenow fragment , biology , oligomer , dna , dna polymerase i , base pair , reverse transcriptase , microbiology and biotechnology , dna synthesis , polymerase , dna polymerase , biochemistry , oligonucleotide , exonuclease , polymerase chain reaction , chemistry , gene , polymer chemistry

Unsubstituted oligodeoxynucleotides, or oligodeoxynucleotides linked to poly-(L)-lysine, when hybridized to a 322 base long template, did not inhibit the production of full length DNA copies by the Klenow fragment of E. coli DNA polymerase I. However, synthesis was inhibited if the cysteamine derivative of the same oligomer was cross-linked to the template via PtII. Truncated products were formed by termination of DNA synthesis a small number of bases upstream from the 5'-end of the cross-linked oligomer. AMV reverse transcriptase behaved similarly but was also slightly inhibited by the hybridized oligomer or its poly-lysine derivative.

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