Regions at the carboxyl end of bacteriophage ø29 protein p6 required for DNA binding and activity in ø29 DNA replication | Zendy

María Jesús Otero | Zendy; Margarita Salas | Zendy

Open Access

Regions at the carboxyl end of bacteriophage ø29 protein p6 required for DNA binding and activity in ø29 DNA replication

Author(s) -

María Jesús Otero,

Margarita Salas

Publication year - 1989

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/17.12.4567

Subject(s) - biology , dna replication , dna , ter protein , microbiology and biotechnology , replication factor c , control of chromosome duplication , replication protein a , bacteriophage , amino acid , eukaryotic dna replication , mutant , origin recognition complex , dna binding protein , dna synthesis , biochemistry , gene , escherichia coli , transcription factor

Series of deletions corresponding to the carboxyl end of the phage phi 29 protein p6 have been constructed and their activity in the initiation of phi 29 DNA replication and their capacity to interact with the phi 29 DNA ends have been studied. Determination of the activity of the deletion mutants in phi 29 DNA replication indicated the dispensability of the 14 carboxy-terminal amino acids of the protein. The activity of protein p6 decreased with deletions from 23 to 39 amino acids and was undetectable when 44 amino acids were removed. A similar behaviour was obtained when the interaction of the mutant proteins with the phi 29 DNA ends was analyzed. These results indicate that the stimulation of phi 29 DNA replication by protein p6 requires a specific binding to the phi 29 DNA ends.

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