Bcefl, a new type IIs restriction endonuclease | Zendy

Pál Venetianer | Zendy; András Orosz | Zendy

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Bcefl, a new type IIs restriction endonuclease

Author(s) -

Pál Venetianer,

András Orosz

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.7.3053

Subject(s) - biology , restriction enzyme , cleavage (geology) , recognition sequence , bacillus cereus , endonuclease , restriction fragment , genetics , restriction map , microbiology and biotechnology , dna , nucleic acid sequence , bacteria , paleontology , fracture (geology)

A new Type IIS restriction endonuclease was identified, partially purified and characterized from a Bacillus cereus subsp. fluorescens strain. The enzyme recognizes the nonpalindromic sequence ACGGC and cleaves at a distance from it. The cleavage appears to occur with a +/- 1 basepair uncertainty. Thus the cleavage and recognition site is as shown below: ACGGC(N)11-13 TGCCG(N)12-14.

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