In vitro transcribed SV4O minichromosomes, as the bulk mlnichromosomes, have a low level of unconstrained negative supercoils | Zendy

Mordechai Choder | Zendy; Yosef Aloni | Zendy

Open Access

In vitro transcribed SV4O minichromosomes, as the bulk mlnichromosomes, have a low level of unconstrained negative supercoils

Author(s) -

Mordechai Choder,

Yosef Aloni

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.3.895

Subject(s) - dna supercoil , minichromosome , biology , topoisomerase , dna , nucleosome , topology (electrical circuits) , plasmid , microbiology and biotechnology , biophysics , genetics , physics , chromatin , dna replication , mathematics , combinatorics

The method for detecting the small fraction (1%) of transcriptionally active SV40 minichromosomes, in the presence of the bulk minichromosomes, (14) has been applied to directly analyze the topology of transcribed and non-transcribed minichromosomal DNA. We show here that DNA of both transcribed and non-transcribed minichromosomes have the same number of supercoils which are constrained by nucleosomes. In addition, minichromosomal DNA of both fractions have a low level of unconstrained supercoils (1-2 extra supercoils) which can be relaxed in vitro by topoisomerase I.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research