Identification of psiB genes of plasmids F and R6-5. Molecular basis for psiB enhanced expression in plasmid R6-5 | Zendy

M. Dutreix | Zendy; A Bäckman | Zendy; Jérôme Célérier | Zendy; M. M. Bagdasanan | Zendy; Suzanne Sommer | Zendy; Adriana Bailone | Zendy; Raymond Devoret | Zendy; M. Bagdasarianh | Zendy

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Identification of psiB genes of plasmids F and R6-5. Molecular basis for psiB enhanced expression in plasmid R6-5

Author(s) -

M. Dutreix,

A Bäckman,

Jérôme Célérier,

M. M. Bagdasanan,

Suzanne Sommer,

Adriana Bailone,

Raymond Devoret,

M. Bagdasarianh

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.22.10669

Subject(s) - biology , plasmid , gene , genetics , identification (biology) , computational biology , microbiology and biotechnology , botany

PsiB protein of plasmid R6-5 inhibits the induction of the SOS pathway. The F sex factor also carries a psiB gene homologous to that of R6-5. Yet, it fails to inhibit SOS induction. In order to solve this difference, we characterized the psiB genes of R6-5 and F. We found that (i) the sequences of the two psiB genes share extensive homology the predicted amino acid sequences of the two proteins differing by 5 residues, (ii) the expression of R6-5 psiB is 4 times higher than F psiB gene, (iii) in plasmid R6-5, a Tn10 transposon upstream from the psiB gene enhances psiB expression. Hence, the F sex factor may be unable to prevent SOS induction for two non-exclusive reasons: (i) F PsiB protein, being slightly different from R6-5, may be less active, (ii) the level of synthesis of F PsiB protein may be insufficient to prevent SOS induction.

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