Characterization of DNA polymerase β mRNA: cell-cycle and growth response in cultured human cells | Zendy

Barbara Z. Zmudzka | Zendy; A. Fomace | Zendy; James M. Collins | Zendy; Samuel H. Wilson | Zendy

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Characterization of DNA polymerase β mRNA: cell-cycle and growth response in cultured human cells

Author(s) -

Barbara Z. Zmudzka,

A. Fomace,

James M. Collins,

Samuel H. Wilson

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.20.9587

Subject(s) - biology , microbiology and biotechnology , cell cycle , dna polymerase , dna , messenger rna , polymerase , dna synthesis , cell culture , genetics , cell , gene

DNA polymerase beta (beta-polymerase) is a housekeeping enzyme involved in DNA repair in vertebrate cells. We used a cDNA probe to study abundance of beta-polymerase mRNA in cultured human cells. The mRNA level in synchronized HeLa cells, representing different stages of the cell-cycle, varied only slightly. Contact inhibited fibroblasts AG-1522 contained the same level of mRNA as growing cells. The steady-state level of mRNA in fibroblasts is equivalent to 6 molecules per cell. The results indicate that the beta-polymerase transcript is "low abundance" and is neither cell-cycle nor growth phase responsive.

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