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Mitochondrial DNA of the yeastKluyveromyces: guanine—cytosine rich sequence clusters
Author(s) -
Antonella RagniniWilson,
Hiroshi Fukuhara
Publication year - 1988
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/16.17.8433
Subject(s) - biology , kluyveromyces marxianus , kluyveromyces lactis , cytosine , yeast , mitochondrial dna , guanine , genetics , nucleic acid sequence , dna , restriction map , genome , palindromic sequence , palindrome , saccharomyces cerevisiae , gene , nucleotide
Mitochondrial DNA from the yeast Kluyveromyces marxianus var. lactis (K.lactis) is a circular molecule of 39 kilobase-pairs. A genetic and physical map was constructed. We found that this genome contained a large number of guanine-cytosine (GC)-rich sequence clusters, many of which are characterized by the presence of SacII restriction sites (CCGCGG). The primary sequence of the GC clusters often showed a palindromic structure. These GC clusters were present in several varieties of K.marxianus, but not in others. The presence of these clusters is a major feature that distinguishes K.lactis strains from those of K.marxianus var. marxianus (including K.fragilis).

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