Sequence of the gene encoding the mitochondrial F1ATPase alpha subunit from Nicotiana plumbaginifolia | Zendy

François Chaumont | Zendy; Marc Boutry | Zendy; Michel Briquet | Zendy; A. Vassarotti | Zendy

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Sequence of the gene encoding the mitochondrial F1ATPase alpha subunit from Nicotiana plumbaginifolia

Author(s) -

François Chaumont,

Marc Boutry,

Michel Briquet,

A. Vassarotti

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.13.6247

Subject(s) - biology , genetics , gene , mitochondrial dna , sequence (biology) , protein subunit , nucleic acid sequence , nicotiana , g alpha subunit , microbiology and biotechnology , solanaceae

A genomic bank of Nicotiana plumbaqinifolia mitochondrial DNA was constructed and probed with the gene encoding the a subunit of maize mitochondrial Fn-APTase (1,2). A 4.7 kb insert was present in the positive clones isolated. After subcloning, a series of progressive deletions were generated. Sequence analysis revealed a region of 1527 nucleotides encoding a polypeptide of 55,162 molecular weight. The amino acid sequence shares respectively 94,3 % et 93,9 % homology with the a subunit sequence of maize (1,2) and Oenothera (3).

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