Anthranilate synthase component II from Hansenula polymorpha

The genomic DNA sequence encoding component II of anthranilate synthase from the methylotrophic yeast Hansenula polymorpha has been identified from published sequence data (1) by homology of the predicted amino acid sequence with that of the corresponding polypeptide from Saccharomyces cerevisiae (2). Ledeboer et al. (1) reported the entire methanol oxidase coding sequence plus 1.3 kb downstream of the termination codon. Within this downstream region I have identified the sequence encoding most of component II of anthranilate synthase; the sequence is incomplete at the C-terminal end. The initiation codon is situated just 170 bp downstream from the methanol oxidase sequence, both proteins being encoded on the same DNA strand. Extensive homology on both sides of the sequence around amino acids 244-247, but in different reading frames indicates a probable error in the published DNA sequence in this region. The predicted amino acid sequence of the anthranilate synthase component II (upper) is shown below, aligned with the S.cerevisiae protein sequence (lower).