Nucleotide sequence of the gene coding for quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus

We recently reported the cloning and characterization of the gene coding for quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus LMD 79.41 (1). In this paper we present the complete nucleotide sequence of this gene as determined by the chain termination method of Sanger et al (2). From the putative start codon to the ochre codon the gene is 2400 bp in lenght, coding for a protein of 86.9 kD. The 140 amino acids long N-terminal part of the protein is highly hydrophobic, suggesting that this domain anchors the protein in the plasma membrane. Putative membrane spanning segments within the hydrophobic domain (hydrophobic regions separated by hydrophilic amino acid residues) are boxed. The translation start site and the possible ribosomal binding site are underlined.