Open AccessCharacterization of response elements for androgens, glucocorticoids and progestins in mouse mammary tumour virus
Author(s) -
Jonathan Ham,
Axel A. Thomson,
Maurice Needham,
Paul Webb,
Malcolm G. Parker
Publication year - 1988
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/16.12.5263
Subject(s) - biology , hormone response element , mouse mammary tumor virus , steroid hormone , steroid , androgen , endocrinology , medicine , glucocorticoid receptor , response element , transcription (linguistics) , glucocorticoid , hormone , palindromic sequence , androgen receptor , receptor , virus , gene , genetics , promoter , gene expression , estrogen receptor , linguistics , philosophy , prostate cancer , cancer , breast cancer , crispr , palindrome
We have characterized steroid response elements in mouse mammary tumour virus (MMTV) by transient transfection. Four partial inverted repeats of the sequence TGTTCT function as response elements for androgen, as well as for glucocorticoid and progestins, although the relative hormone inductions mediated by each oligonucleotide were different. Mutational analysis of the left half of the palindrome showed that a perfect dyad symmetry is not required for optimum activity as a steroid response element. To investigate potential interactions between steroid receptors and transcription factors we have analysed the minimum sequence requirements for a hormone response. Interestingly, a single 15 bp steroid response element and a TATA box are sufficient for steroid inductions. When the distance between the two elements was increased by up to two turns of the helix the hormone induction initially increased and then gradually declined with no obvious periodicity.