Thermodynamic studies of base pairing involving 2,6-diaminopurine | Zendy

Chaejoon Cheong | Zendy; Ignacio Tinoco | Zendy; André Chollet | Zendy

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Thermodynamic studies of base pairing involving 2,6-diaminopurine

Author(s) -

Chaejoon Cheong,

Ignacio Tinoco,

André Chollet

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.11.5115

Subject(s) - base pair , oligonucleotide , duplex (building) , biology , dna , base (topology) , pairing , base sequence , nucleic acid thermodynamics , nucleic acid denaturation , crystallography , stereochemistry , combinatorics , physics , genetics , mathematics , chemistry , mathematical analysis , superconductivity , quantum mechanics

The thermal stabilities of oligodeoxyribonucleotide duplexes containing 2,6-diaminopurine (D) matched with each of the four normal DNA bases were determined by optical melting techniques. Comparison of optical melting curves yielded relative stabilities for the D-containing standard base pairs in an otherwise identical base-pair sequence. The D:T pair was found to be more stable than the A:T pair in dC3DG3:dC3TG3, as stable as the A:T in dCT3DT3G:dCA3TA3G, and less stable than the A:T in dCA3DA3G:dCT7G. The order of stabilities for X:Y in the DNA duplex dCA3XA3G:dCT3YT3G is: (A:T) greater than (T:D) congruent to (D:T) greater than or equal to (T:A) greater than (C:D) congruent to (D:A) congruent to (D:G) greater than or equal to (D:C) congruent to (G:D) congruent to (D:D) greater than or equal to (A:D). Implications of these results for design of DNA oligonucleotide probes are discussed.

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