Import of several tRNAs from the cytoplasm into the mitochrondria in beanPhaseolus vulgaris | Zendy

Laurence MaréchalDrouard | Zendy; JacquesHenry Weil | Zendy; Pierre Guillemaut | Zendy

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Import of several tRNAs from the cytoplasm into the mitochrondria in beanPhaseolus vulgaris

Author(s) -

Laurence MaréchalDrouard,

JacquesHenry Weil,

Pierre Guillemaut

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.11.4777

Subject(s) - biology , phaseolus , transfer rna , cytoplasm , protein biosynthesis , mitochondrion , mitochondrial dna , rna , aminoacylation , biochemistry , genetics , microbiology and biotechnology , gene , botany

Four P. Vulgaris mitochondrial tRNA(Leu) species have been shown to be nuclear encoded. These mt tRNAs(Leu) can be used for in vitro protein synthesis. We found that the sequences of P. vulgaris mitochondrial and cytoplasmic tRNAs(Leu)(NAG) are identical except for a post-transcriptional modification occurring at position 18 (Gm in mt tRNA(Leu) instead of G in cyt tRNA(Leu], as in the case of mt and cyt tRNAs(Leu)(NAA) already sequenced. This post-transcriptional modification has also been found in two other bean mt tRNA(Leu) species, but not in P. vulgaris cytoplasmic tRNA(Leu) species that we have purified so far. Furthermore, comparison of the 2-D polyacrylamide gel electrophoretic patterns of tRNAs eluted from bean mt tRNA-mtDNA and mt tRNA-nDNA hybrids revealed at least 8 mt tRNAs coded for by the nuclear genome.

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