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A cDNA library was prepared from a mixture of genomic RNA segments 7, 8, and 9 of the OSU strain of porcine rotavirus (serotype 5). Genoraic RNA was Isolated from virus which had been plaque-purified a minimum of three times 1n HA104 cells. Multiple cDNA libraries were constructed using several distinct RNA preparations, and each was screened for the presence of full-length clones. Individual cDNA clones were characterized using both chemical and dideoxy sequencing procedures. For the gene segment encoding the serotype-specific glycoprotein VP7, sequence data was also obtained by direct dideoxy sequencing of genomic RNA using oligonucleotide primers.

Nucleotide sequence of porcine rotavirus (OSU strain) gene segments 7, 8, and 9