Nucleotide sequence of porcine rotavirus (OSU strain) gene segments 7, 8, and 9 | Zendy

Keith E. Rushlow | Zendy; Alistair R. McNab | Zendy; Ken E. Olson | Zendy; F Maxwell | Zendy; Ian H. Maxwell | Zendy; Gary L. Stiegler | Zendy

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Nucleotide sequence of porcine rotavirus (OSU strain) gene segments 7, 8, and 9

Author(s) -

Keith E. Rushlow,

Alistair R. McNab,

Ken E. Olson,

F Maxwell,

Ian H. Maxwell,

Gary L. Stiegler

Publication year - 1988

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/16.1.367

Subject(s) - biology , nucleic acid sequence , gene , genetics , strain (injury) , rotavirus , nucleotide , sequence (biology) , virology , base sequence , reoviridae , microbiology and biotechnology , virus , anatomy

A cDNA library was prepared from a mixture of genomic RNA segments 7, 8, and 9 of the OSU strain of porcine rotavirus (serotype 5). Genoraic RNA was Isolated from virus which had been plaque-purified a minimum of three times 1n HA104 cells. Multiple cDNA libraries were constructed using several distinct RNA preparations, and each was screened for the presence of full-length clones. Individual cDNA clones were characterized using both chemical and dideoxy sequencing procedures. For the gene segment encoding the serotype-specific glycoprotein VP7, sequence data was also obtained by direct dideoxy sequencing of genomic RNA using oligonucleotide primers.

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