Open AccessMolecular cloning and sequence analysis of a mouse Y chromosome RNA transcript expressed in the test is
Author(s) -
Colin E. Bishop,
D. Hatat
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.7.2959
Subject(s) - biology , microbiology and biotechnology , complementary dna , rna , y chromosome , genetics , chromosome 22 , sequence analysis , cloning (programming) , chromosome , molecular cloning , cdna library , gene , computer science , programming language
Using a Y specific probe (pY353/B) taken from a flow sorted mouse Y chromosome library we have identified a family of RNA transcripts encoded by the Y chromosome. These transcripts which are approximately 1.3 Kb in length are present in testis PolyA+ RNA but can not be detected in either male liver, spleen, kidney, brain, heart or lung tissues. Isolation and sequence analysis of a corresponding cDNA shows it to contain a potential coding sequence of 696bp. These data show that the Y chromosome is transcriptionally active in the adult mouse testis.