Molecular cloning and sequence analysis of a mouse Y chromosome RNA transcript expressed in the test is | Zendy

Colin E. Bishop | Zendy; D. Hatat | Zendy

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Molecular cloning and sequence analysis of a mouse Y chromosome RNA transcript expressed in the test is

Author(s) -

Colin E. Bishop,

D. Hatat

Publication year - 1987

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/15.7.2959

Subject(s) - biology , microbiology and biotechnology , complementary dna , rna , y chromosome , genetics , chromosome 22 , sequence analysis , cloning (programming) , chromosome , molecular cloning , cdna library , gene , computer science , programming language

Using a Y specific probe (pY353/B) taken from a flow sorted mouse Y chromosome library we have identified a family of RNA transcripts encoded by the Y chromosome. These transcripts which are approximately 1.3 Kb in length are present in testis PolyA+ RNA but can not be detected in either male liver, spleen, kidney, brain, heart or lung tissues. Isolation and sequence analysis of a corresponding cDNA shows it to contain a potential coding sequence of 696bp. These data show that the Y chromosome is transcriptionally active in the adult mouse testis.

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