Gene cluster for streptomycin biosynthesis inStreptomyces griseus: nucleotide sequence of three genes and analysis of transcriptional activity | Zendy

Jürgen Distler | Zendy; Andrea Ebert | Zendy; Kambiz Mansouri | Zendy; Klaus Pissowotzkri | Zendy; Michael Stockmann | Zendy; Wolfgang Piepersberg | Zendy

Open Access

Gene cluster for streptomycin biosynthesis inStreptomyces griseus: nucleotide sequence of three genes and analysis of transcriptional activity

Author(s) -

Jürgen Distler,

Andrea Ebert,

Kambiz Mansouri,

Klaus Pissowotzkri,

Michael Stockmann,

Wolfgang Piepersberg

Publication year - 1987

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/15.19.8041

Subject(s) - biology , streptomyces griseus , gene cluster , gene , genetics , nucleic acid sequence , sequence (biology) , sequence analysis , nucleotide , streptomyces , bacteria

Three streptomycin (SM) production genes from Streptomyces griseus clustered around aphD, the major resistance gene, have been sequenced: strB, coding for an aminocyclitol amidinotransferase, ORF5 (strR), a putative regulatory gene, and ORF1 (strD), possibly coding for a hexose nucleotidylating enzyme. Three promoters and at least five, partially overlapping, transcripts have been identified by S1 mapping and Northern blot experiments. aphD, the resistance gene, is transcribed from two promoters. One of them, located inside the strR gene, seems to be constitutive and the other is switched on later in the growth phase. The late transcripts cover the resistance gene (aphD) and a regulatory gene (strR) which controls the expression of strB.

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