Open AccessAn anonymous single copy clone (p1,2-2) from a flow sorted human chromosomes 1 and 2 library (D1S33)
Author(s) -
Abraham Grossman,
Linda A. Cannizzaro,
Larry A. Warmoth,
Roger N. Rosenberg
Publication year - 1987
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/15.14.5904
Subject(s) - biology , genetics , clone (java method) , work flow , dna , industrial engineering , engineering
SOURCE AND DESCRIPTION OF CLONE: pi,2-2, a 1.8 Kb EcoRI fragment was subcloned in pBR322, derived from a genomic library in Charon 21A which was constructed from sorted by flow cytometry human chromosome 1 and 2. POLYMORPHISM: EcoRI indentifies two alleles with DNA fragments at either 1.8 Kb (Al) or 1.0 and 0.3 Kb (A2), Hind III identifies two alleles with DMA fragments at either 5.0 (Bl) or 8.0 Kb (B2). FREQUENCIES: EcoRI: Al all ele 73% Hind III: Bl allele 58% based on the study of 15 unrelated individuals. NOT POLYMORPHIC FOR: TaqI
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