c-mycprotein can be substituted for SV40 T antigen in SV40 DNA replication | Zendy

Sanae M.M. IguchiAriga | Zendy; Teru Itani | Zendy; Masamitsu Yamaguchi | Zendy; Hiroyoshi Ariga | Zendy

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c-mycprotein can be substituted for SV40 T antigen in SV40 DNA replication

Author(s) -

Sanae M.M. IguchiAriga,

Teru Itani,

Masamitsu Yamaguchi,

Hiroyoshi Ariga

Publication year - 1987

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/15.12.4889

Subject(s) - plasmid , biology , transfection , microbiology and biotechnology , dna replication , antigen , sv40 large t antigen , origin of replication , gene , dna , raji cell , replication factor c , cell culture , virology , control of chromosome duplication , genetics

Replicating activity of SV40 origin-containing plasmid was tested in human cells as well as in monkey CosI cells. All the plasmids possessing SV40 ori sequences could replicate, even in the absence of SV40 T antigen, in human HL-60 and Raji cells which are expressing c-myc gene at high level. The copy numbers of the replicated plasmids in these human cells were 1/100 as high as in monkey CosI cells which express SV40 T antigen constitutively. Exactly the same plasmids as the transfected original ones were recovered from the Hirt supernatant of the transfected HL-60 cells. Furthermore, replication of the SV40 ori-containing plasmids in HL-60 cells was inhibited by anti-c-myc antibody co-transfected into the cells. These results indicate that the c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication.

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