A new restriction endonudease fromSpirulina platensis | Zendy

Masahide Kawamura | Zendy; Masaki Sakakibara | Zendy; Teruo Watanabe | Zendy; Keiko Kita | Zendy; Nobutsugu Hiraoka | Zendy; Akira Ôbayashi | Zendy; Masamichi Takagi | Zendy; Keiji Yano | Zendy

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A new restriction endonudease fromSpirulina platensis

Author(s) -

Masahide Kawamura,

Masaki Sakakibara,

Teruo Watanabe,

Keiko Kita,

Nobutsugu Hiraoka,

Akira Ôbayashi,

Masamichi Takagi,

Keiji Yano

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.5.1985

Subject(s) - isoschizomer , biology , haeiii , restriction enzyme , bacteriophage , pbr322 , dna , foki , lambda phage , microbiology and biotechnology , ecori , spirulina (dietary supplement) , genetics , recognition sequence , cleave , subspecies , biochemistry , plasmid , restriction fragment length polymorphism , escherichia coli , gene , polymerase chain reaction , polymorphism (computer science) , raw material , gene expression , ecology , genotype , dna methylation , paleontology

Three restriction endonucleases, Sp1I, Sp1II and Sp1III have been purified partially from Spirulina platensis subspecies siamese and named. Sp1I cleaves bacteriophage lambda DNA at one site, phi X 174 RF DNA at two sites, but does not cleave pBR322 DNA. This enzyme recognizes the sequence 5'CGTACG3' 3'GCATCG5' and cuts the site indicated by the arrows. Sp1II is an isoschizomer of Tth111I and Sp1III is an isoschizomer of HaeIII.

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