Molecular cloning and nucleotide sequence of the streptavidin gene | Zendy

Carlos E. Argaraña | Zendy; Irwin D. Kuntz | Zendy; Steven Birken | Zendy; Richard Axel | Zendy; Charles R. Cantor | Zendy

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Molecular cloning and nucleotide sequence of the streptavidin gene

Author(s) -

Carlos E. Argaraña,

Irwin D. Kuntz,

Steven Birken,

Richard Axel,

Charles R. Cantor

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.4.1871

Subject(s) - biology , nucleic acid sequence , gene , oligonucleotide , peptide sequence , microbiology and biotechnology , molecular cloning , coding region , sequence analysis , genomic dna , genetics , homology (biology) , protein primary structure , cloning (programming) , dna , genomic library , streptavidin , biotin , computer science , programming language

Using synthetic oligonucleotides as probes we have cloned the streptavidin gene from a genomic library of Streptomyces avidinii. Nucleotide sequence analysis indicated that a 2 Kb DNA-fragment contained the entire coding region, a signal peptide region and the 3' and 5' flanking regions of the gene. The deduced amino acid sequence shows several interrupted blocks of homology with the amino acid sequence of chicken egg-white avidin. Analysis of the secondary structure suggests a high content of beta-structure in both proteins and considerable overall structural similarity between them.

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