Regulatory DNA elements localized remotely upstream from the drug-metabolizing cytochrome P-450c gene | Zendy

Atsuko ujisawa-Sehara | Zendy; Kazuhiro ogawa | Zendy; Chikako Nishi | Zendy; Yoshiaki FujiiKuriyama | Zendy

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Regulatory DNA elements localized remotely upstream from the drug-metabolizing cytochrome P-450c gene

Author(s) -

Atsuko ujisawa-Sehara,

Kazuhiro ogawa,

Chikako Nishi,

Yoshiaki FujiiKuriyama

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.3.1465

Subject(s) - biology , chloramphenicol acetyltransferase , regulatory sequence , microbiology and biotechnology , gene , tata box , transcription (linguistics) , upstream activating sequence , dna , genetics , gene expression , enhancer , reporter gene , promoter , linguistics , philosophy

We have investigated regulatory DNA elements in the expression of the drug metabolizing P-450c gene of rats. After combining the 5' flanking and upstream untranslated regions of the isolated P-450c gene with structural gene for chloramphenicol acetyltransferase (CAT), the fusion genes were transfected into cultured cells (Hepa-1 and L cells) for the assay of transient expression of CAT activity. CAT activity was expressed inducibly in response to 3-methylcholanthrene only in Hepa-1 cells. At least three regions containing regulatory DNA elements were indentified; one, which is present in the sequence from -44b to -0.2kb immediately upstream of the TATA box, functions in the basal level of transcription, and the other two which were located in the sequence from -0.8kb to -1.0kb and from -1.0kb to -6.3kb, enhance in combination, transcription in response to inducers in a manner independent of their orientation.

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