A human gastric carcinoma contains a single mutated and an amplified normal allele of the Ki-rasoncogene | Zendy

Johannes L. Bos | Zendy; Matty Verlaan-de Vries | Zendy; C. J. Marshall | Zendy; G.H. Veeneman | Zendy; Jacques H. van Boom | Zendy; A.J. van der Eb | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A human gastric carcinoma contains a single mutated and an amplified normal allele of the Ki-rasoncogene

Author(s) -

Johannes L. Bos,

Matty Verlaan-de Vries,

C. J. Marshall,

G.H. Veeneman,

Jacques H. van Boom,

A.J. van der Eb

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.3.1209

Subject(s) - biology , allele , microbiology and biotechnology , transfection , oncogene , oligonucleotide , mutation , genetics , cancer research , malignancy , dna , cell culture , cell , gene , cell cycle

The DNA from various human tumors and tumor cell lines was screened for the presence of mutated ras oncogenes with synthetic oligonucleotide probes, as well as with the NIH/3T3 cell transfection assay. Among the various mutations found we discovered two novel Ki-ras mutations in codon 12: gly to ala and gly to ser. A gastric carcinoma was found to possess a single mutated Ki-ras allele (gly-12 to ser), as well as a 30-50 fold amplified normal allele. This implies that two activating steps must have occurred in this malignancy.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research