Does 5S RNA fromE. colihave a pseudoknotted structure? | Zendy

H. Ulrich Göringer | Zendy; Rolf Wagner | Zendy

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Does 5S RNA fromE. colihave a pseudoknotted structure?

Author(s) -

H. Ulrich Göringer,

Rolf Wagner

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.18.7473

Subject(s) - conformational isomerism , biology , rna , nucleotide , stacking , molecule , nucleic acid structure , stereochemistry , helix (gastropod) , crystallography , biochemistry , chemistry , ecology , organic chemistry , snail , gene

Chemical modification and limited enzymatic hydrolysis on isolated E. coli 5S RNA have provided informations on the secondary- and tertiary structure compatible with pseudoknotted structures for the A- and B-conformers of the molecule. Changes in the accessibility and reactivity of nucleotides in loop C and at the stem of helix IV in two different 5S RNA conformers are highly suggestive for interactions between bases C35 to C37 with G105 to G107 for the A-form and C38 to U40 and A94 to G96 with additional interactions of C35, C37 with G98 and G100 for the B-form. In both cases the molecules are folded forming pseudoknots and two quasi--continuous double stranded helices with coaxial stacking. The two structures are in perfect agreement with the biochemical data concerning the stability of the molecule and the chemical reactivities of individual nucleotides of the 5S RNA A- and B-conformers.

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