Isolation and characterization of the gene encoding the testis specific histone protein H2B-2 from the sea urchinLytechinus pictus | Zendy

Zhi-Chun Lai | Zendy; Geoffrey Childs | Zendy

Open Access

Isolation and characterization of the gene encoding the testis specific histone protein H2B-2 from the sea urchinLytechinus pictus

Author(s) -

Zhi-Chun Lai,

Geoffrey Childs

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.17.6845

Subject(s) - biology , histone h2b , tata box , gene , histone h1 , genetics , histone , histone h2a , sap30 , histone methyltransferase , microbiology and biotechnology , gene expression , promoter

We have cloned and characterized the gene encoding a sperm specific H2B-2 histone subtype from the sea urchin L. pictus. The gene is not clustered with any other histone genes. However, it resembles other histone genes in many respects. The gene contains no intervening sequences or polyadenylation signals. Like other histone genes it contains the conserved 3' hairpin loop sequence and CAAGAAAGA box necessary for the processing of the 3' end of the histone transcript. Upstream of the gene in addition to the TATA box, and two copies of inverted CCAAT Boxes is a conserved sequence element found in many H2B genes. Unlike most histone genes, the H2B-2 gene is expressed in a sex and tissue specific manner only in testis. The sperm H2B-2 subtype encoded by this gene is 142 amino acids in length. The larger size of this H2B protein is accounted for by residues in the N-terminus of the protein consisting of a series of pentapeptide repeats typical of this histone subtype.

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