Drosophila ARSs contain the yeastARSconsensus sequence and a replication enhancer | Zendy

John Mills | Zendy; Alan J. Kingsman | Zendy; Susan M. Kingsman | Zendy

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Drosophila ARSs contain the yeastARSconsensus sequence and a replication enhancer

Author(s) -

John Mills,

Alan J. Kingsman,

Susan M. Kingsman

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.16.6633

Subject(s) - biology , autonomously replicating sequence , drosophila melanogaster , plasmid , subcloning , genetics , enhancer , origin of replication , drosophila (subgenus) , yeast , dna replication , drosophilidae , p element , dna , gene , transcription factor

A number of restriction fragments that function as autonomously replicating sequences (ARSs) in yeast have been isolated from Drosophila melanogaster DNA. The behaviour in yeast of plasmids containing Drosophila ARS elements was studied and compared to that exhibited by the archetypal yeast ARS-1 plasmid. ARS functions were localised by subcloning and BAL-31 deletion analysis. These studies demonstrated the structural and functional complexity of Drosophila ARSs. Each Drosophila ARS element has at least two domains, one essential for replication (the replication sequence, RS) and a second (the replication enhancer, RE) which is essential for maximum function of the RS. The RS of three Drosophila ARSs was shown to contain a sequence identical to an 11 bp yeast ARS consensus sequence (5' A/T TTTATPuTTT A/T 3'). These observations lend support to the hypothesis that heterologous ARS elements may be of biological significance.

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