Floral tissue ofPetunia hybrida(V30) expresses only one member of the chalcone synthase multigene family | Zendy

Ronald Koes | Zendy; Cornelis Spelt | Zendy; H. J. Reif | Zendy; Peter J. M. van den Elzen | Zendy; E. Veltkamp | Zendy; Jos Mol | Zendy

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Floral tissue ofPetunia hybrida(V30) expresses only one member of the chalcone synthase multigene family

Author(s) -

Ronald Koes,

Cornelis Spelt,

H. J. Reif,

Peter J. M. van den Elzen,

E. Veltkamp,

Jos Mol

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.13.5229

Subject(s) - biology , petunia , chalcone synthase , petal , gene , complementary dna , genetics , cloning (programming) , microbiology and biotechnology , sequence analysis , coding region , clone (java method) , southern blot , gene family , genome , botany , gene expression , computer science , programming language

Twenty independent, petal-specific chalcone synthase (CHS) cDNA clones have been isolated from Petunia hybrida variety Violet 30 (V30). Sequence analysis shows that the largest of these clones contains the entire coding sequence. Using this clone in Southern blot analysis reveals the presence of multiple CHS gene copies in the genome of Petunia hybrida V30. Hybridization and sequence analysis of the CHS cDNA clones shows that they are all copied from a single mRNA species. This indicates the presence of only one transcriptionally active CHS gene in petals. Finally we report the identification, cloning and partial characterization of this gene.

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