The nudeotide sequence of the gene for γ-glutamylcysteine synthetase ofEscherichia coli | Zendy

Kunihiko Watanabe | Zendy; Yoshinori Yamano | Zendy; Kousaku Murata | Zendy; Akira Kimura | Zendy

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The nudeotide sequence of the gene for γ-glutamylcysteine synthetase ofEscherichia coli

Author(s) -

Kunihiko Watanabe,

Yoshinori Yamano,

Kousaku Murata,

Akira Kimura

Publication year - 1986

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/14.11.4393

Subject(s) - biology , escherichia coli , gene , sequence (biology) , genetics , peptide sequence , escherichia coli proteins , enterobacteriaceae , microbiology and biotechnology

The nucleotide sequence of the gsh I gene for gamma-glutamylcysteine synthetase(GSH I) of Escherichia coli B has been determined. The gsh I structural gene contains 1557 bases in length and predicted a ploypeptide of 518 amino acids with a calculated molecular weight of 58,251. The value is in good agreement with that obtained from gel filtration and SDS/PAGE of GSH I. The initiation codon 5 bp downstream of putative Shine-Dalgarno sequence was an unusual TTG, which encoded methionine. For transcription, two sets of consensus promoter signals(-10 and -35 regions) overlapping each other were identified. The terminator signal shows the favored stem-loop structure with an adequate free energy delta G = -22.80 kcal/mol.

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