Open AccessVarious rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family
Author(s) -
Philippe Fort,
L. Marty,
Marc Piechaczyk,
S.El Sabrouty,
Christian Dani,
P Jeanteur,
JeanMarie Blanchard
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.5.1431
Subject(s) - glyceraldehyde 3 phosphate dehydrogenase , biology , complementary dna , nucleic acid sequence , microbiology and biotechnology , coding region , messenger rna , dehydrogenase , primer extension , nucleotide , nuclease , glyceraldehyde , primer (cosmetics) , biochemistry , genetics , gene , enzyme , chemistry , organic chemistry
We have isolated and sequenced a full-length cDNA clone encoding rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C.1.2.1.12). The entire mRNA is 1269 nucleotides long exclusive of poly(A) and contains respectively 71 and 196 bases of 5' and 3' non-coding regions. Primer extension as well as S1 nuclease protection experiments clearly established that a single (or at least a highly prominent) GAPDH mRNA species is expressed in all rat tissues examined. This sequence allowed the determination of the hitherto unknown primary structure of rat GAPDH which is 333 aminoacids long. Comparison between GAPDH sequences from rat, man and chicken revealed a high degree of sequence conservation at both nucleotide and protein levels.