Open AccessMethylation of thymine residues during oligonucleotide synthesis
Author(s) -
Xiang Gao,
Barbara L. Gaffney,
M. Senior,
R.R. Riddle,
Roger A. Jones
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.2.573
Subject(s) - oligonucleotide , biology , methylation , thymine , alkylation , oligonucleotide synthesis , dna methylation , biochemistry , dna , microbiology and biotechnology , gene , gene expression , catalysis
Thymine residues in an oligodeoxyribonucleotide are subject to methylation at N3 by the internucleotide methyl phosphotriester linkages. This alkylation occurs most rapidly in the presence of a strong base such as DBU, but also takes place, at a much slower rate, during oligonucleotide synthesis.
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