Synthesis and hybridization of dodecadeoxyribonucleotides containing a fluorescent pyridopyrimidine deoxynucleoside | Zendy

H. Inoue | Zendy; Akihiro Imura | Zendy; Eiko Ohtsuka | Zendy

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Synthesis and hybridization of dodecadeoxyribonucleotides containing a fluorescent pyridopyrimidine deoxynucleoside

Author(s) -

H. Inoue,

Akihiro Imura,

Eiko Ohtsuka

Publication year - 1985

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/13.19.7119

Subject(s) - biology , duplex (building) , guanine , nucleoside , fluorescence , pyrimidine , nucleotide , hydrogen bond , pairing , dna , biochemistry , microbiology and biotechnology , stereochemistry , molecule , gene , chemistry , physics , quantum mechanics , superconductivity , organic chemistry

Partially self-complementary dodecadeoxyribonucleotides containing a fluorescent nucleoside, 3-beta-D-2'-deoxyribofuranosyl-2, 7-dioxopyrido[2, 3-d]pyrimidine (pyridopyrimidine deoxynucleoside, dF) were synthesized by the phosphotriester solidphase method. A dodecanucleotide d(GGGAAFGTTCCC) pairing the analog and guanine at the centre of the chain showed a higher melting temperature than the corresponding G-C paired duplex. A similar comparison between A-T and A-F suggested that weaker hydrogen bonds exist when adenine and pyridopyrimidine residues are paired.

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