The ribosomal RNA promoter ofAcanthamoeba castellaniidetermined by transcription in a cellfree system | Zendy

Preecha Kownin | Zendy; Calvin T. lida | Zendy; Sheryl BrownShimer | Zendy; Marvin R. Paule | Zendy

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The ribosomal RNA promoter ofAcanthamoeba castellaniidetermined by transcription in a cellfree system

Author(s) -

Preecha Kownin,

Calvin T. lida,

Sheryl BrownShimer,

Marvin R. Paule

Publication year - 1985

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/13.17.6237

Subject(s) - biology , transcription (linguistics) , ribosomal rna , acanthamoeba , rna , dna , general transcription factor , mutant , microbiology and biotechnology , promoter , genetics , gene , gene expression , linguistics , philosophy

The DNA sequences required for faithful initiation of ribosomal RNA transcription were determined. BAL-31 digestion was used to modify the rDNA template by introducing deletions from its 3'- and 5'-ends. The resulting mutant DNAs were tested for template activity individually or in competition with wild type utilizing an in vitro transcription system from Acanthamoeba castellanii. The results identify the sequence extending from -31 to +8 to be absolutely required for transcription. In addition; when the region between -47 and -32 is left intact, transcription is augmented.

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