On the fidelity of DNA polymerase α: the influence of α-thio dNTPs, Mn2+and mismatch repair | Zendy

Axel Rienitz | Zendy; Frank Große | Zendy; Helmut Blöcker | Zendy; Ronald Frank | Zendy; Gerhard Krauss | Zendy

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On the fidelity of DNA polymerase α: the influence of α-thio dNTPs, Mn²⁺and mismatch repair

Author(s) -

Axel Rienitz,

Frank Große,

Helmut Blöcker,

Ronald Frank,

Gerhard Krauss

Publication year - 1985

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/13.15.5685

Subject(s) - biology , dna , dna polymerase , genetics , polymerase , microbiology and biotechnology , dna repair , computational biology

The phi X174am16 revertant system has been used to investigate the influence of alpha-thio-dNTPs and of Mn2+ on the fidelity of the 9S DNA polymerase alpha from calf thymus. Upon substituting dGTP by alpha-thio-dGTP during the in vitro replication, a nearly tenfold decrease in the frequency of G:G and G:T mispairs is observed. The formation of all other mispairs is not changed in the presence of the corresponding alpha-thio-dNTP. Mn2+ at concentrations of 0.5 mM does not influence the frequencies of the mispairs. The expression rate of errors formed during in vitro replication in the (-) strand has been determined for all mispairs detectable in the phi Xam16 system. The (-) strand expression of G:T, T:T and C:T mismatches is about 50%, whereas for A:G, G:G and C:A mismatches it is clearly below 50%. We conclude that the different base-base mismatches are repaired with different efficiencies.

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