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Sequence and codon recognition of bean mitochondria and chloroplast tRNAsTrP: evidence for a high degree of bomology
Author(s) -
Laurence Maréchal,
Pierre Guillemaut,
Jean-Michel Grienenberger,
G. Jeannin,
JacquesHenry Weil
Publication year - 1985
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/13.12.4411
Subject(s) - biology , mitochondrion , chloroplast , homology (biology) , genetics , transfer rna , genetic code , chloroplast dna , gene , mitochondrial dna , tryptophan , microbiology and biotechnology , rna , amino acid
Bean mitochondria and chloroplast tRNAsTrp, purified by RPC-5 chromatography and two-dimensional gel electrophoresis, have been sequenced using post-labeling techniques. The high degree of sequence homology between bean mitochondria and chloroplast tRNAsTrp shows that these two tRNAs are coded for by closely related genes which have probably evolved from a common ancestor gene. The anticodon of bean mitochondria tRNATrp is CmCA, which can recognize UGG (the codon for tryptophan in the universal code) and is complementary neither to UGA (which codes for tryptophan in mammalian and yeast mitochondria) nor to CGG (which could be a tryptophan codeword in plant mitochondria).

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