Molecular amplification and purification of theE. colt recCgene product | Zendy

Ian D. Hickson | Zendy; Karen E. Atkinson | Zendy; L Hutton | Zendy; Alan E. Tomkinson | Zendy; Peter T. Emmerson | Zendy

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Molecular amplification and purification of theE. colt recCgene product

Author(s) -

Ian D. Hickson,

Karen E. Atkinson,

L Hutton,

Alan E. Tomkinson,

Peter T. Emmerson

Publication year - 1984

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/12.9.3807

Subject(s) - biology , plasmid , gene , microbiology and biotechnology , gene product , gene expression , lambda phage , genetics , bacteriophage , escherichia coli

The level of recC gene expression has been analysed using Mud(bla lac) fusions to the recC promoter. The constitutive level of expression is very low and remains so even under SOS inducing conditions. The recC gene product has been amplified by harnessing the gene to the phage lambda leftward promoter in a plasmid. From cells harbouring this plasmid, RecC protein, which represented approximately 6% of the total cellular protein, was purified to electrophoretic homogeneity.

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