Eight new restriction endonucleases fromHerpetosiphon giganteus– divergent evolution in a family of enzymes | Zendy

Manfred Kröger | Zendy; Gerd Hobom | Zendy; H. R. Schütte | Zendy; Hubert Mayer | Zendy

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Eight new restriction endonucleases fromHerpetosiphon giganteus– divergent evolution in a family of enzymes

Author(s) -

Manfred Kröger,

Gerd Hobom,

H. R. Schütte,

Hubert Mayer

Publication year - 1984

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/12.7.3127

Subject(s) - biology , restriction enzyme , genetics , enzyme , evolutionary biology , dna , biochemistry

Characterization of eight restriction endonucleases isolated from five strains of Herpetosiphon giganteus is described. HgiCI from strain Hpg9 recognizes and cleaves the degenerate sequence: GGPyPuCC, producing 5'-hexanucleotide protruding ends. Endonucleases HgiBI, HgiCII and HgiEI are isoschizomers of AvaII; HgiCIII and HgiDII are isoschizomers of SalI; and HgiDI and HgiGI are isoschizomers of AcyI. Based upon their closely related and in part overlapping recognition specificities a close evolutionary relationship is proposed for all known Hgi restriction endonucleases.

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