The complele DNA sequence and regulatory regions of theBacillus licheniformis spoOHgene | Zendy

N. V. S. Ramakrishna | Zendy; E Dubnau | Zendy; Issar Smith | Zendy

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The complele DNA sequence and regulatory regions of theBacillus licheniformis spoOHgene

Author(s) -

N. V. S. Ramakrishna,

E Dubnau,

Issar Smith

Publication year - 1984

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/12.4.1779

Subject(s) - biology , gene , genetics , bacillus licheniformis , transcription (linguistics) , rna polymerase , bacillus subtilis , promoter , nucleic acid sequence , base pair , open reading frame , dna , microbiology and biotechnology , sigma factor , consensus sequence , rna , peptide sequence , gene expression , linguistics , philosophy , bacteria

We have determined the sequence of a 1228 base-pair cloned DNA fragment from Bacillus licheniformis capable of specifically complementing mutations in the spoOH gene, which is required for the early stage of sporulation in B. subtilis. The sequence has only one long open reading frame consisting of 168 codons. In vivo and in vitro transcription mapping studies indicate the size of complementary RNA to be around 1 kb with the 5' initiation site at base 79 and the 3' termination site in the area of base 1138. This indicates the presence of a 5' untranslated RNA and a fairly long 3' extension. The promoter sequence of this gene is 5'TATAAT3' at -10, and 5'TTGACG3' at -35, a typical E. coli-like promoter sequence, and is transcribed in vitro specifically only by RNA polymerase containing delta 55 and not delta 37-containing holoenzyme.

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