Diadenosine 5′ ,5′ ″-P1, P4in devdoplng embryos ofArtemia | Zendy

Alexander G. McLennan | Zendy; Mark Prescott | Zendy

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Diadenosine 5′ ,5′ ″-P¹, P⁴in devdoplng embryos ofArtemia

Author(s) -

Alexander G. McLennan,

Mark Prescott

Publication year - 1984

Publication title -

nucleic acids research

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/12.3.1609

Subject(s) - brine shrimp , biology , embryo , dna replication , dna synthesis , artemia salina , dna , microbiology and biotechnology , biochemistry , ecology , medicine , toxicity

Diadenosine 5',5'"-P1,P4-tetraphosphate (Ap4A) has been detected in cysts and developing embryos of the brine shrimp Artemia in amounts 10(4)-10(6) times lower than that of the guanine analogue, Gp4G. The unexpectedly high level of Ap4A in dormant cysts of 2.37 pmol/10(6) cells can be reduced to 0.03 pmol/10(6) cells by decapsulation and storage in saturated NaCl. When development is reinitiated, the Ap4A content of the decapsulated embryos undergoes a rapid 125 -fold increase, reaching a maximum of 3.79 pmol/10(6) cells at the point of emergence when DNA replication begins. If replication is delayed by hypoxia, the Ap4A level is adjusted in order to reach the same maximum value when replication finally begins. As replication proceeds, the level of Ap4A declines again. Unlike mammalian cells, Ap4A in Artemia is less metabolically labile than ATP. These results are consistent with the suggested role of Ap4A in the initiation of DNA synthesis.

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