Molecular cloning of a human immunoglobulin λ chain variable sequence | Zendy

Yuji Tsujimoto | Zendy; C M Croce | Zendy

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Molecular cloning of a human immunoglobulin λ chain variable sequence

Author(s) -

Yuji Tsujimoto,

C M Croce

Publication year - 1984

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/12.22.8407

Subject(s) - biology , microbiology and biotechnology , complementary dna , nucleic acid sequence , southern blot , primer (cosmetics) , molecular cloning , peptide sequence , gene , genetics , organic chemistry , chemistry

We have cloned a human V lambda cDNA sequence from an Ig lambda-producing human Burkitt lymphoma cell line (BL2) by taking advantage of a cloned constant region gene as a primer for cDNA synthesis instead of an oligo(dT) primer. The amino acid sequence deduced from the nucleotide sequence of V lambda clones is highly related to that of the NEW V lambda protein of subgroup I. Southern blot hybridization of human DNAs with the V lambda I probe showed at least 12 hybridizing V lambda fragments. These fragments are amplified in K562 cells which derive from a case of chronic myelogenous leukemia and contain an amplified c-abl oncogene and amplified C lambda sequences.

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