The wild-type nucleotide sequence of therpoBC-attenuator region ofEscherichia coliDNA, and its implications for the nature of therifd18 mutation | Zendy

Brian A. Morgan | Zendy; Elaine Kellett | Zendy; Richard S. Hayward | Zendy

Open Access

The wild-type nucleotide sequence of therpoBC-attenuator region ofEscherichia coliDNA, and its implications for the nature of therif^d18 mutation

Author(s) -

Brian A. Morgan,

Elaine Kellett,

Richard S. Hayward

Publication year - 1984

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/12.13.5465

Subject(s) - biology , rpob , genetics , attenuator (electronics) , dna , nucleic acid sequence , escherichia coli , microbiology and biotechnology , nucleotide , dna sequencing , lambda phage , gene , bacteriophage , physics , 16s ribosomal rna , attenuation , optics

To investigate the possibility that the unusual dominant rifampicin-resistance characteristic of the rifd18 allele of E. coli rpoB is due to a secondary, regulatory mutation, we have determined the nucleotide sequence of a 1.1 Kbp wild-type DNA fragment, including the transcriptional attenuator and translational start-site of rpoB. We have also re-investigated the previously published sequences of this region in lambda rifd18 and lambda rifd47 DNA. Our results indicate that all three sequences are identical, and reveal some errors in the published data. We discuss the basis of dominance of rifd18.

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