Conformational analysis of a ribopentandeolde tetraphosphate in aqueous solution. A two-dimensional NMR study at 500 MHZ1 | Zendy

Peter P. Lankhorst | Zendy; G. Wille | Zendy; Jacques H. van Boom | Zendy; Conelis Altona | Zendy; Comdis A.G. Haasnoot | Zendy

Open Access

Conformational analysis of a ribopentandeolde tetraphosphate in aqueous solution. A two-dimensional NMR study at 500 MHZ¹

Author(s) -

Peter P. Lankhorst,

G. Wille,

Jacques H. van Boom,

Conelis Altona,

Comdis A.G. Haasnoot

Publication year - 1983

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/11.9.2839

Subject(s) - conformational isomerism , nuclear overhauser effect , nuclear magnetic resonance spectroscopy , spectroscopy , ring (chemistry) , crystallography , two dimensional nuclear magnetic resonance spectroscopy , proton , stacking , population , stereochemistry , coupling constant , nuclear magnetic resonance , molecule , physics , chemistry , demography , organic chemistry , quantum mechanics , particle physics , sociology

The 30 ribose proton resonances of the pentaribonucleoside tetraphosphate m6(2)AUm6(2)AUm6(2)A have been assigned unequivocally by means of spin-echo-correlated spectroscopy, 2D J-resolved spectroscopy and Nuclear Overhauser difference spectroscopy, carried out at 500 MHz. A detailed comparison of the conformational properties of the title compound with its constituent fragments m6(2)AUm6(2)AU, m6(2)AUm6(2)A, m6(2)AU and the relevant monomers is given. Chemical shift data indicate the existence of a doubly "bulged out" conformer, in which the two interior U-fragments are not involved in regular nearest neighbour stacking interactions. The coupling constants of the ribose-ring are interpreted in terms of the N/S equilibrium, and population distributions along the backbone angles beta and gamma are presented. The combined data suggest a strong similarity between the 5'-terminal triplets in m6(2)AUm6(2)AUm6(2)A, m6(2)AUm6(2)AU and m6(2)AUm6(2)A2.

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