Localization of low-melting regions in phage T7 DNA | Zendy

Victor I. Lyamichev | Zendy; Igor G. Panyutin | Zendy; Dmitry I. Cherny | Zendy; Yu. L. Lyubchenko | Zendy

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Localization of low-melting regions in phage T7 DNA

Author(s) -

Victor I. Lyamichev,

Igor G. Panyutin,

Dmitry I. Cherny,

Yu. L. Lyubchenko

Publication year - 1983

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/11.7.2165

Subject(s) - biology , dna , restriction enzyme , restriction map , microbiology and biotechnology , nucleic acid denaturation , dna sequencing , restriction fragment , nucleic acid thermodynamics , gel electrophoresis , genetics , nucleic acid sequence , base sequence

Specific fragmentation of T7 DNA at glyoxal-fixed denatured regions by the S1 endonuclease followed by restriction analysis made it possible to localize four low-melting regions in phage T7 DNA. These regions have the following coordinates:0.5-1.2;14.8+/-0.3;46.3+/-0.5; 98.4+/-0.3 (in T7 DNA length units). The location of the low-melting regions was refined by means of electron-microscopic denaturation mapping and gel electrophoresis of partially denatured DNA. The obtained localization of the low-melting regions is consistent with the available data on the sequence of T7 DNA. The map of low-melting regions was compared with the genetic map of T7 DNA.Images

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