Structure and expression of cloned murine IFN-α genes | Zendy

Gray D. Shaw | Zendy; Werner Boll | Zendy; Hideharu Taira | Zendy; Ned Mantei | Zendy; Peter Lengye | Zendy; Charles Weissmann | Zendy

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Structure and expression of cloned murine IFN-α genes

Author(s) -

Gray D. Shaw,

Werner Boll,

Hideharu Taira,

Ned Mantei,

Peter Lengye,

Charles Weissmann

Publication year - 1983

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/11.3.555

Subject(s) - biology , gene , genetics , gene expression , microbiology and biotechnology , molecular cloning , cloning (programming) , computer science , programming language

The mouse has an interferon-alpha (MuIFN-alpha) gene family containing at least four, and likely more than ten members. A segment of mouse chromosomal DNA and cDNAs encoding murine alpha IFNs have been cloned, and the sequence of two MuIFN-alpha DNAs determined. No intron was found in the chromosomal gene. The two coding sequences produced biologically active IFN when expressed in monkey cells under the control of an SV40 promoter, and in E.coli under the control of the ampicillinase promoter. MuIFN-alpha 1 had no detectable activity on human cells, while MuIFN-alpha 2 was 20% as active on human as on mouse cells.

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